Thermo Scientific™ D/E (Dey-Engley) Neutralizing Broth (Dehydrated)

Neutralize chemical disinfectants to distinguish bacteriostatic and bactericidal activity in environmental sampling procedures using D/E (Dey-Engley) Neutralizing Broth. The medium can neutralize a range of chemicals including mercurial, iodine or chlorine preparations, quaternary ammonium compounds, formaldehyde and glutaraldehyde.

• Easy to Identify: Bromocresol Purple is used as an indicator for the fermentation of dextrose by shifting from purple to yellow at acidic pH.
• Easy to differentiate: Subculture all tubes of D/E Neutralizing Broth to appropriate media such as D/E Agar to observe growth (viable microorganisms).
  • Bacteriostatic substance – Growth from negative D/E Neutralizing Broth tubes.
  • Bactericidal substance – No growth from negative D/E Neutralizing Broth tubes.
• Recommended in the Compendium of Methods for the Microbiological Examination of Foods by the American Public Health Association (APHA) for environmental sampling².

This medium contains peptone and yeast extract that provide a source of nitrogen, carbon, and vitamins. Dextrose provides energy and sodium thiosulfate is incorporated to neutralize chlorine and iodine. Polysorbate 80 neutralizes phenols and formalin. Sodium thioglycolate neutralizes mercurials. Sodium bisulfite neutralizes formaldehyde and glutaraldehyde. Lecithin neutralizes quaternary ammonium compounds.. This medium is suitable for environmental sampling where neutralization of the chemical is important to determine its bactericidal activity.

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