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Thermo Scientific™ Pierce™ Protein L Plus Agarose

Product Code. 10178063 Shop All Thermo Scientific Products
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Quantity:
2 mL
Unit Size:
2mL
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Description

Purify antibodies containing kappa light chains or perform immunoprecipitation with this high-capacity Protein L resin on beaded agarose.

Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce High-Capacity Protein L Agarose, a high-capacity Protein L beaded agarose resin.

Pierce Protein L Plus Agarose is a high-capacity affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains. Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies. The resin consists of recombinant Protein L immobilized on crosslinked 6% beaded agarose (CL-6B) using a stable, leach-resistant chemistry. The agarose beads can be regenerated and reused multiple times when stored properly.

Highlights:

  • Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains

  • Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods

  • Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield

  • High capacity – “Plus” variety of Pierce Protein L Agarose has a dense load of immobilized Protein L, providing a binding capacity of 10 to 20mg human IgG/mL resin

Properties of crosslinked 6% beaded agarose (CL-6B):

  • Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
  • Average Particle Size: 45 to 165 microns
  • Exclusion Limit: 10,000 to 4,000,000 daltons
  • Maximum Linear Velocity: 30cm per hour
  • Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather
  • than the pressure drop across the column.)
TRUSTED_SUSTAINABILITY

Specifications

Product Line Pierce™
Column Type Agarose Resin, Affinity
Format Bottle
Stationary Phase Protein L
Purification Target Ig Antibodies
Content And Storage Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Quantity 2 mL

For Research Use Only. Not for use in diagnostic procedures.

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