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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, Midi kit for gel extraction and PCR clean up
PCR clean up and gel extraction – the two in one kit.
Brand: Macherey-Nagel™ 740986.20
This item is not returnable.
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Description
NucleoSpin™ Gel and PCR Clean-up Midi
- Two applications in one kit – one buffer with optimal performance for both applications
- High recoveries for fragments down to 50 bp
- Binding buffer with pH indicator
- Separate buffers for single stranded DNA or SDS containing samples available
- Suitable for all gel buffer systems (e.g., TAE, TBE)
- Ensures complete removal of contaminations such as: nucleoties, primers, enzymes, mineral oil, PCR additives, detergents, and dyes
- Typical downstream applications: cloning, sequencing, PCR, restriction analysis
Specifications
| Room temperature (18-25 °C) | |
| Silica Membrane Technology | |
| Binding capacity: 75 μg | |
| Centrifuge | |
| NucleoSpin Gel and PCR Clean-up Midi Columns, buffers | |
| A260/A280: 1.75 - 1.85 | |
| 25 min/6 preps |
| Midi Spin Column | |
| Agarose and Polyacrylamide Gels, PCR Reaction Mixture | |
| Elution volume: 200 - 400 μL | |
| PCR clean-up and gel extraction | |
| 20 Preps | |
| <4 mL PCR reaction mixture, <4 g agarose gel | |
| Typical recovery: 70 - 95 % |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- NucleoSpin Gel and PCR Clean-up Midi(20)
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
Supplemental information
- MIXTURE LIST-Contains : guanidium thiocyanate
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