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Macherey-Nagel™ NucleoBond™ RNA/DNA 400, Maxi kit with anion exchange technology
Anion exchange chromatography for nucleic acids of highest integrity.
Marca: Macherey-Nagel™ 740651
Descrizione
NucleoBond™ RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80μg or 400μg ultra pure total RNA. A special manufacturing process guarantees RNase-free handling. Due to the superior resolving power of NucleoBond™ anion exchanger contaminations are washed away, and RNA and genomic DNA can be eluted separately.
Specific references:
M. Jiang et al. , J. Virol. Methods
38
(1992) 205T. Roitsch et al. , Plant Physiol.
108
(1995) 285D. Godt et al. , J. Plant Physiol.
146
(1995) 231R. Ehness et al. , The Plant Cell
9
(1997) 1825S. P. Hale et al. , Science
276
(1997) 1250Kit components: NucleoBond™ RNA/DNA 80 - NucleoBond™ AXR 80 columns, all required buffers. NucleoBond™ RNA/DNA 400 - NucleoBond™ AXR 400 columns, all required buffers.
- Anion-exchange technology
- Typical RNA yield: 70μg to 300μg from cultured cells 30μg to 150μg from tissue 50μg to 200μg from bacteria
- Sample material: eukaryotic cultured cells, tissue, bacteria/yeast cells
- Fragment size: 50bp to 300kb
- Binding capacity: 80μg for NucleoBond RNA/DNA 80 400μg for NucleoBond RNA/DNA 400
- Preparation time: 1.5 hr. to 2. 5 hr.
- Format: gravity-flow columns - midi or maxi
Specifica
10 Preps | |
RNA/DNA 400 |
Sicurezza e movimentazione
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
- PERICOLO A LUNGO TERMINE PER L'AMBIENTE ACQUATICO Cronico 3
- Lesione oculare grave/irritazione oculare Categoria 2
- Liquido infiammabile Categoria 3
- Corrosione/irritazione della pelle Categoria 2
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
- H302-Nocivo se ingerito.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
- H412-Nocivo per gli organismi acquatici con effetti di lunga durata.
Informazioni supplementari
- MIXTURE LIST-Contiene: ethanol, guanidiniumthiocyanate, tritonx-100
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