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Invitrogen™ PureLink™ Quick Gel Extraction Kit
Description
Includes
Gel Solubilization Buffer (L3)
Wash Buffer (W1)
Elution Buffer (E5); (10 mM Tris-HCl, pH 8.5)
Quick Gel Extraction Columns
Wash Tubes
Recovery Tubes
The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ∼30 minutes using PureLink™ silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum.
Advantages of using PureLink™ Quick Gel Extraction Kit:
- Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points
- Complete the procedure in ∼30 minutes
- Easily purify DNA fragments from 40 bp to 10 kb from gels
- Obtain high recovery of DNA fragments
- Bind and purify up to 15 μg DNA with one column
- Purify DNA fragments that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling
Note: The PureLink™ Quick Gel Extraction Kit is not designed to purify supercoiled plasmid DNA or genomic DNA from agarose gels. Only linear DNA fragments may be purified from gels using this kit.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | PureLink Elution Tubes For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Isolation Technology | Silica Spin Column |
| Sample Type | Agarose Gels, Gel Samples |
| Elution Volume | 50 μL |
| Final Product Type | DNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| Green Features | Less hazardous, sustainable packaging |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Label or Dye | Ethidium Bromide, SYBR Safe |
| Quantity | 50 Preps |
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Safety and Handling
- 0.12 LT PURELINK GEL EXTRACTION KIT
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Skin corrosion/irritation Category 1 C
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H412-Harmful to aquatic life with long lasting effects.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Thiocyanic acid, compound with guanidine
For Research Use Only. Not for use in diagnostic procedures.
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