Invitrogen™ Silencer™ Negative Control No. 1 siRNA

Description

Silencer Negative Control No. 1 siRNA is a bioinformatically designed, non-targeting siRNA used as a negative control siRNA. It distinguishes specific effects of siRNA-mediated gene silencing from non-specific procedural effects, ensuring minimal off-target effects and providing a reliable baseline for comparison. Validated through extensive testing, it has minimal impact on gene expression, cell proliferation, viability, and morphology, making it essential for accurate results in human, mouse, and rat cells.

Negative controls, such as non-targeting siRNAs, are critical for distinguishing the specific effects of siRNA-mediated gene silencing from non-specific effects caused by the experimental procedure itself. Silencer negative control No. 1 is designed to ensure minimal off-target effects, providing a robust baseline for comparing the results of siRNA-treated samples. This negative control siRNA shares the same chemical modifications as other Silencer siRNAs.

Features of Silencer Negative Control siRNAs include:

• No significant sequence similarity to human mouse, or rat genes
• Minimal effects on cell proliferation and viability
• Matching Silencer™ siRNA chemistry

Validated through extensive testing, including microarray analysis and multi-parametric cell-based assays, Silencer Negative Control No. 1 has been proven to have minimal impact on gene expression, cell proliferation, viability, and morphology. This makes it an essential tool for researchers aiming to achieve accurate and consistent phenotypic data in human, mouse, and rat cells.

What are Silencer siRNAs?

Silencer siRNAs are designed for potency and specificity using a proprietary algorithm. These unmodified siRNAs are cost-effective siRNAs with guaranteed silencing.

Quality control

Each Silencer siRNA is synthesized and purified in state-of-the-art facilities to meet the highest quality standards. Our rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. We also assess each annealed siRNA by gel electrophoresis to ensure proper strand annealing. The result is a premium-quality siRNA that is purified and ready for use.