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  5. Cytiva HiTrap™ Protein A HP Prepacked Columns

Cytiva HiTrap™ Protein A HP Prepacked Columns

Product Code. p-3667283 Shop All Cytiva Products
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Quantity:
1 x 5 mL
2 x 1 mL
5 x 1 mL
5 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
Each
Pack of 2
Pack of 5
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

  • Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
  • For use with syringe, pump or chromatography system such as KTA design or FPLC System
  • Binds 20mg human IgG/mL medium
  • Stable over pH 3 to 9 for use and 2 to 10 for cleaning
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns

Media
  • Ligand: Native protein A
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 9

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity 20 mg/mL IgG Medium
Flow Rate <20 mL/min.
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 8°C, 20% Ethanol
Quantity 5 x 5 mL
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