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  5. Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Product Code. 10298714 Shop All Cytiva Products
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Quantity:
2500 cm2 Membrane
For 5000 cm2 membrane
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.

DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

  • Hybridization stringency can be controlled by elevating temperatures up to 75°C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2 or 5000cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage 2°C to 8°C
Description AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.
For Use With (Application) Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
Includes Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
Product Type AlkPhos Direct Labeling Module
Quantity 2500 cm2 Membrane

Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin sensitiser Category 1
  • Germ cell mutagenicity Category 2
  • Carcinogenicity Category 1B
  • Specific target organ toxicity after single exposure Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H317-May cause an allergic skin reaction.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
  • H370-Causes damage to organs.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : methanol, formaldehyde
  • UNKNOWN ACUTE-100 per cent of the mixture consists of component(s) of unknown acute toxicity.
  • UNKNOWN AQUATIC-Contains 100% of components with unknown hazards to the aquatic environment.
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