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Thermo Scientific™ GeneJET Gel Extraction Kit
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Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.
The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.
Highlights
- Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
- Fast: procedure takes only 15 minutes
- Convenient: spin columns are capped and assembled with collection tubes
Applications
Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
- FastDigest or conventional restriction digestion
- Automated fluorescent or radioactive sequencing
- PCR
- In situ hybridization
- Labeling
- Cloning
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Spin Column |
| Isolation Technology | Spin Column |
| Sample Type | Gel |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Product Line | GeneJET |
| Product Type | Gel Extraction Kit |
| Quantity | 50 Preps |
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Safety and Handling
- GeneJET Gel Extraction Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Skin corrosion/irritation Category 1 C
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H412-Harmful to aquatic life with long lasting effects.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : thiocyanic acid
For Research Use Only. Not for use in diagnostic procedures.
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